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KMID : 0624620220550030148
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BMB Reports
2022 Volume.55 No. 3 p.148 ~ p.153
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Pulsed electromagnetic field potentiates etoposide-induced MCF-7 cell death
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Woo Sung-Hun
Kim Bo-Hee
Kim Sung-Hoon
Jung Byung-Chul
Lee Yong-Heum
Kim Yoon-Suk
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Abstract
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Etoposide is a chemotherapeutic medication used to treat various types of cancer, including breast cancer. It is established that pulsed electromagnetic field (PEMF) therapy can enhance the effects of anti-cancer chemotherapeutic agents. In this study, we investigated whether PEMFs influence the anti-cancer effects of etoposide in MCF-7 cells and determined the signal pathways affected by PEMFs. We observed that co-treatment with etoposide and PEMFs led to a decrease in viable cells compared with cells solely treated with etoposide. PEMFs elevated the etoposide-induced PARP cleavage and caspase-7/9 activation and enhanced the etoposide-induced down-regulation of survivin and up-regulation of Bax. PEMF also increased the etoposide-induced activation of DNA damage-related molecules. In addition, the reactive oxygen species (ROS) level was slightly elevated during etoposide treatment and significantly increased during co-treatment with etoposide and PEMF. Moreover, treatment with ROS scavenger restored the PEMF-induced decrease in cell viability in etoposide-treated MCF-7 cells. These results combined indicate that PEMFs enhance etoposide-induced cell death by increasing ROS induction?DNA damage?caspase-dependent apoptosis.
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KEYWORD
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DNA damage, Etoposide, MCF-7 cell death, Pulsed electromagnetic field (PEMF), Reactive oxygen species (ROS)
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